Skripsi

Isolation and characterization of endophytic fungi from andong leaves (cordyline fruticosa) with antimalarial potential through inhibition of ?-hematin formation / Cresensia Jessica Wanda Rossyana Puteri

Abstrak
Malaria is a disease caused by Plasmodium parasites that live within human blood cells. The increasing incidence of parasite resistance to antimalarial drugs has become a major global health concern. Therefore the discovery of new alternative treatments is essential to replace and suppress such resistance. One plant that shows potential as an antimalarial agent is the leaf of the andong plant (Cordyline fruticosa). However the exploration of natural products for malaria treatment requires abundant natural resources which poses a risk of overharvesting and potential extinction of C. fruticosa. Endophytic fungi are known to produce bioactive compounds that are like those of their host plants and may exhibit comparable biological activities. The aim of this study is to isolate and characterize endophytic fungi from C. fruticosa leaves obtain fungal extracts identify the phytochemical content of each isolate evaluate the antimalarial potential of each isolate through inhibition of beta -hematin formation and genetically identify the most promising endophytic fungal species with antimalarial potential. The research was conducted in a laboratory setting and consisted of seven stages (1) isolation and purification of endophytic fungi from Cordyline fruticosa leaves (2) macroscopic and microscopic characterization of the pure endophytic fungal isolates (3) cultivation of the pure isolates on rice medium (4) extraction of fungal compounds using ethyl acetate through maceration (5) qualitative phytochemical screening of the fungal extracts (6) in-vitro antimalarial activity assay through beta -hematin formation inhibition and (7) molecular identification of the most potential endophytic fungal species as an antimalarial agent. The results of the study showed that five pure endophytic fungal isolates were successfully obtained from Cordyline fruticosa leaves labeled with the code JDAUM (Jamur Daun Andong). Based on the extraction results the highest yield was obtained from the JDAUM5 extract while the lowest yield was from JDAUM3. Furthermore phytochemical screening revealed that the JDAUM5 extract contained secondary metabolites including alkaloids flavonoids phenolics and terpenoids. In the antimalarial activity assay JDAUM5 extract demonstrated the highest antimalarial activity. Data analysis showed a significant difference in inhibition between JDAUM1 and JDAUM4 extracts JDAUM1 and JDAUM5 extracts as well as between JDAUM1 and the positive control ( ) with p-values lt 0.05. In addition a significant difference was also found between extract concentrations of 5 mg/mL and 0.01 mg/mL with a p-value lt 0.0083. Based on molecular identification the JDAUM5 isolate was identified as Aspergillus flavus.

Informasi Detail

DDC

SKRIPSI DIGITAL

Prodi

Universitas Negeri Malang. Program Studi Bioteknologi, 2025.

Deskripsi Fisik

3 hlm. : ilus.

Bahasa

No Reg

3926/RS/25

Edisi

Skripsi (Sarjana)--Universitas Negeri Malang. 2025

Subjek

1. ENDOPHYTIC FUNGI - CORDYLINE FRUTICOSA - ANTIMALARIAL

Pembimbing

1. Dr. Muh. Ade Artasasta, S.si; Dwi Listyorini, M.si, D.sc

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