Skripsi

Selection and optimization of incubation time, ph, and inducer concentration in lipase enzyme production by lipolytic bacteria from the biotechnology laboratory collection of universitas negeri malang / Puan Maharani

Abstrak
Lemuru fish oil (Sardinella lemuru) is known to contain high levels of omega-3 fatty acids. The omega-3 content in lemuru fish oil can be enhanced through the catalytic activity of lipase enzymes. This study aims to identify potential lipase-producing isolates and determine the optimal conditions for lipase enzyme production including incubation time pH and inducer concentration using local bacterial strains from East Java. The research was conducted in a laboratory setting with the following scope (1) Hemolysis test to assess the pathogenicity of each isolate (2) Primary screening of lipolytic activity using selective agar medium (3) Secondary screening based on lipolytic activity in production medium and (4) Optimization of incubation time pH and inducer concentration using the One Factor at a Time (OFAT) method. The results showed that all isolates exhibited pathogenic characteristics. Screening on selective medium indicated that two isolates demonstrated lipolytic activity as evidenced by the formation of yellow zones. Among the tested isolates one isolates exhibited the highest specific lipase activity as determined by UV/VIS spectrophotometry. The highest specific activity of lipase from the isolate was obtained at 48 hours of incubation pH 7 and 1% inducer concentration.

Informasi Detail

DDC

SKRIPSI DIGITAL

Prodi

Universitas Negeri Malang. Program Studi Bioteknologi, 2025.

Deskripsi Fisik

xiii, 5 hlm. : ilus.

Bahasa

No Reg

4058/RS/25

Edisi

Skripsi (Sarjana)--Universitas Negeri Malang. 2025

Subjek

1. PRODUKSI ENZIM - ENZIM LIPASE
2. PRODUKSI ENZIM - LIPASE BAKTERI
3.ENZYME PRODUCTION - LIPASE ENZYME

Pembimbing

1. Dr. Evi Susanti, S.si, M.si

Lampiran Berkas