Skripsi

Detection of cevd (citrus exocortis viroid) in citrus plants of bsip jestro collection based on pcr (polymerase chain reaction) / Dwi Endah Wulandari

Abstrak
Citrus is one of the most strategic horticultural commodity plants to be developed both for fresh consumption and for processed products. Several factors inhibit the quality of citrus plants including abiotic and biotic stress factors. Abiotic stress is a sub-optimal condition resulting from non-living (abiotic) factors that limit plant growth and yield. Biotic stress is a sub-optimal condition caused by living (biotic) factors such as insects bacteria viruses diseases and weeds which limit plant growth and yield an example of this is systemic disease due to viroid infection such as Citrus Exocortis Viroid (CEVd). Currently another method being developed is the Polymerase Chain Reaction (PCR)-based method. This research aims to determine the presence of CEVd disease in citrus plants using PCR with commercial kits and infrastructure available at BSIP Jestro. The results showed that detection of CEVd disease in citrus plants using PCR with 18s rRNA and CEVd primers available at BSIP Jestro can be successfully carried out at BSIP Jestro. Based on the results obtained of six tested plant samples several samples tested positive for CEVd.

Informasi Detail

DDC

SKRIPSI DIGITAL

Prodi

Universitas Negeri Malang. Program Studi Bioteknologi, 2025.

Deskripsi Fisik

4 hlm. : ilus.

Bahasa

No Reg

4276/RS/25

Edisi

Skripsi (Sarjana)--Universitas Negeri Malang. 2025

Subjek

1. JERUK - CEVD - PCR
2. ORANGE - CEVD - PCR

Pembimbing

1. Dr. Evi Susanti, S.si, M.si

Lampiran Berkas