Skripsi

Uji potensi dan optimasi konsentrasi lignoselulosa beberapa limbah pertanian sebagai sumber karbon dalam produksi enzim b-glukosidase Phanerochaete chrysosporium / Wuri Damayanti

Abstrak
Damayanti W. Potential Test and Optimization Concentration of Lignocellulosic from Various Agricultural Waste as Carbon Source on Enzyme 946 -glucosidase Production from Phanerochaete chrysosporium. Undergraduated Department of Chemistry Faculty of Mathematics and Natural Science State University of Malang. Advisors (1) Evi Susanti S.Si. M.Si. (2) Dr. Suharti M.Si. Kata Kunci 946 -glukosidase lignocellulosic agricultural waste carbon source Phanerochaete chrysosporium 12288 12288 12288 12288 Enzyme 946 -glucosidase is the enzyme that catalyzes hydrolysis reaction cellobiosa to glucose. Enzyme 946 -glucosidase production from P.chrysosporium is strongly influenced by the type of carbon source in the media production. The aimed of this researched were examine scientific information about (1) the most potential carbon source among lignocellulosic agricultural waste corn stover bagasse rice husk rice straw and sawdust in 946 -glucosidase production from P.chrysosporium (2) optimization concentration of the most potential lignocellulosic agricultural waste as carbon source in 946 -glucosidase production from P.chrysosporium 12288 12288 12288 12288 This research are laboratory that conducted in Departmen of Chemistry State University of Malang and Microbiology Laboratory State University of Brawijaya Malang. The phase of this research are (1) sample preparation (lignocellulosic) as carbon source are rice straw rice husk corn stover bagasse and sawdust (2) rejuvenation pure culture of P.chrysosporium (3) manufacture of P.chrysosporium spore suspension (4) 946 -glukosidase production from P.chrysosporium using lignocellulosic as carbon source (5) determination the optimum concentration of the most potential carbon source to produce 946 -glukosidase enzyme from P.chrysosporium. Lignocellulosic concentration used are 0 0 3 0 5 0 7 1 0 dan 1 2 % (b/v). Selection the most potential carbon source and determination the optimum concentration based on the level of 946 -glucosidase activity. The 946 -glucosidase activity was determined by reduction of the amount of sugar produced after the reaction. Reducing sugar content was determined by using the DNS method. 12288 12288 12288 12288 The result of this research showed that rice straw rice husk corn stover bagasse and sawdust have the potential as carbon source on 946 -glucosidase production from P.chrysosporium. The use of waste corn stover bagasse rice straw rice husk and sawdust at a concentration of 1 % can produce consecutive the highest 946 -glucosidase activity 2 169 U/ mL 4 853 U/ mL 12 132 U/ mL 5 00 U / mL and 6 691 U/ mL. The rice straw is the most potential lignocellulosic waste as carbon source on 946 -glukosidase production from P.chrysosporium. The rice straw can produce the highest 946 -glukosidase activity at 1% as big as 12 132 U/ mL. 12288 12288 12288 12288

Informasi Detail

DDC

Rs 547.782 DAM u

Prodi

Universitas Negeri Malang. Program Studi Kimia, 2014.

Deskripsi Fisik

viii, 50 lembar : il., tab. ; 30 cm

Bahasa

Indonesia

No Reg

04431/KI/14

Edisi

Skripsi (Sarjana)-- Universitas Negeri Malang, 2014

Subjek

1. LIGNOSELULOSA - POTENSI
2. LIGNOSELULOSA - KONSENTRASI

Pembimbing

1. Susanti ; 2. SUharti

Lampiran Berkas